http://washingtonlab.org/blog Wed, 26 May 2010 01:25:33 +0000 http://wordpress.org/?v=2.2 en # 17 The chlorophyll derivative chlorin e6 enhances the electrical response of the rabbit eye to light: a study in biological vision enhancement http://washingtonlab.org/blog/?p=51 http://washingtonlab.org/blog/?p=51#comments Fri, 09 Apr 2010 17:51:43 +0000 admin http://washingtonlab.org/blog/?p=51 Jinfeng Qu, Li Ma, Yardana Kaufman, and Ilyas Washington (Submitted, 2010).Purpose: To determine whether the porphyrin chlorin e6 can safely enhance the electrical response of the rabbit eye to light.

Methods: Electroretinogram (ERG) a- and b-wave amplitudes in response to green, blue, amber, red and deep red test flashes were compared for rabbits before and after administration of chlorin e6. Light and chlorin e6 induced retinal pigment epithelium (RPE) cell toxicity was measured by the firefly luciferase assay in cell cultures. 

Results: After administration of chlorin e6 ERG amplitudes were enhanced, particularly in response to red light. For deep red light, we measured up to a three-fold enhancement in b-wave amplitude. Chlorin and light showed negligible toxicity to RPE cells in culture at concentrations studied.

Conclusions: Chlorin e6 may safely be used to enhance the electrical response of the rabbit eye to light. Given the clinical use of chlorin e6, further studies aimed at answering whether porphyrins can enhance vision in humans are warranted.

]]> http://washingtonlab.org/blog/?feed=rss2&p=51 Research Coverage http://washingtonlab.org/blog/?p=50 http://washingtonlab.org/blog/?p=50#comments Tue, 06 Apr 2010 23:25:52 +0000 admin http://washingtonlab.org/blog/?p=50

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]]> http://washingtonlab.org/blog/?feed=rss2&p=50 # 16 Coenzyme Q10 in the human retina http://washingtonlab.org/blog/?p=48 http://washingtonlab.org/blog/?p=48#comments Tue, 06 Apr 2010 22:53:07 +0000 admin http://washingtonlab.org/blog/?p=48 Qu J, Kaufman Y, Washington I.            Invest Ophthalmol Vis Sci. 2009 Apr;50(4):1814-8.   To determine the concentration of coenzyme Q10 (CoQ10) in the human retina. METHODS: Eye tissues were lyophilized and exhaustively extracted with heptane. The extracts were analyzed for CoQ10 by high-performance liquid chromatography (HPLC). RESULTS: The average concentration of CoQ10 in the retina was 42+/-11 nanomoles/g dry retina for donors younger than 30 years of age and 24+/-13 nanomoles/g dry retina for donors older than 80 years of age. The average concentrations of CoQ10 in the choroid was 27+/-16 nanomoles/g dry choroid for donors younger than 30 age and 18+/-11 nanomoles/g dry choroid for donors older than 80. CONCLUSIONS: CoQ10 levels in the retina can decline by approximately 40% with age. This decline may have two consequences: a decrease in antioxidant ability and a decrease in the rate of ATP synthesis in the retina and, as such, this decline may be linked to the progression of macular degeneration. 

]]> http://washingtonlab.org/blog/?feed=rss2&p=48 Discover Magazine Research Coverage http://washingtonlab.org/blog/?p=45 http://washingtonlab.org/blog/?p=45#comments Tue, 06 Apr 2010 22:21:43 +0000 admin http://washingtonlab.org/blog/?p=45 discovermag.jpg

]]> http://washingtonlab.org/blog/?feed=rss2&p=45 # 15 Chlorophyll Derivatives as Visual Pigments for Super Vision in the Red http://washingtonlab.org/blog/?p=35 http://washingtonlab.org/blog/?p=35#comments Mon, 19 May 2008 04:56:09 +0000 admin http://washingtonlab.org/blog/?p=35 Washington, Iyas; Zhou, Jilin; Jockusch, Steffen; Turro, Nicholas J.; Nakanishi, Koji; Sparrow Janet R.  Photochemical and Photobiological Sciences, 2007, 6, 775.

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]]> http://washingtonlab.org/blog/?feed=rss2&p=35 # 14 Strategy for Reversing Resistance to a Single Anti-Cancer Agent in Human Prostate and Pancreatic Carcinomas http://washingtonlab.org/blog/?p=34 http://washingtonlab.org/blog/?p=34#comments Mon, 19 May 2008 04:55:26 +0000 admin http://washingtonlab.org/blog/?p=34 Lebedeva, Irina V.; Washington, Ilyas; Sarkar, Devanand; Clark, Jennifer A.; Fine, Robert L.; Dent, Paul; Curiel, David T., Turro, Nicholas J.; Fisher Paul B.  Proc. Natl. Acad. Sci. U. S. A., 2007, 104 (9) 3484-3489.  

]]> http://washingtonlab.org/blog/?feed=rss2&p=34 #13 An Accessory Chromophore in Red Vision http://washingtonlab.org/blog/?p=33 http://washingtonlab.org/blog/?p=33#comments Mon, 19 May 2008 04:54:39 +0000 admin http://washingtonlab.org/blog/?p=33 Isayama, Tomoki; Alexeev, Dmitriy; Clint, Makino; Washington, Ilyas; Koji Nakanishi; Turro, Nicholas J.  Nature, 2006, 443, 649.

]]> http://washingtonlab.org/blog/?feed=rss2&p=33 # 12 Superoxidation of Retinoic Acid http://washingtonlab.org/blog/?p=32 http://washingtonlab.org/blog/?p=32#comments Mon, 19 May 2008 04:53:54 +0000 admin http://washingtonlab.org/blog/?p=32 Washington, Ilyas; Turro, Nicholas J.; Nakanishi, Koji Photchemistry and Photobiology, 2006, 82(6), 1394–1397.

]]> http://washingtonlab.org/blog/?feed=rss2&p=32 # 11 Superoxidation of Bisretinoids http://washingtonlab.org/blog/?p=31 http://washingtonlab.org/blog/?p=31#comments Mon, 19 May 2008 04:53:01 +0000 admin http://washingtonlab.org/blog/?p=31 Washington, Ilyas; Jockusch, Steffen; Itagaki, Yasuhiro; Turro, Nicholas J.; Nakanishi, Koji  Angew. Chem. Int. Ed. 2005, 117(43), 7259-7262.

]]> http://washingtonlab.org/blog/?feed=rss2&p=31 #10 Temperature and Solvent Control of the Stereoselectivity in the Reactions of Singlet Oxygen with Oxazolidinone-Substituted Enecarbamates http://washingtonlab.org/blog/?p=30 http://washingtonlab.org/blog/?p=30#comments Mon, 19 May 2008 04:52:21 +0000 admin http://washingtonlab.org/blog/?p=30 Poon, Thomas; Sivaguru, J.; Franz, Roberto; Jockusch, Steffen; Martinez, Claudia; Washington, Ilyas; Adam, Waldemar; Inoue, Yoshihisa; Turro, Nicholas J.   J. Am. Chem. Soc. 2004, 126(34), 10498-10499.   

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